Nucleotides in the Catechol Amine Granules of the Adrenal Medulla

Publisher Website
Google Scholar
Abstract
: The work was carried out to determine whether the catechol amines in the adrenal medulla from birds and mammals are stored together with adenosinephosphates in the same way as previously found in the cow. The aniine granules of the adrerial medulla from fowl, goose, cat and goat were isolated by means of density gradient centrifugation. As in the cow no nucleotides other than adenosinephosphates were found (ion exchange and paper chromatography, high voltage paper electrophoresis). No purine or pyrimidine bases or nucleotides were demonstrable. In all animals examined ATP was predominant, but ADP and AMP were always present. The relative proportions of the three phosphates varied rather markedly, however. In spite of this, amines and adenosinephosphatefi were regularly present in practically equivalent amounts.