Haemoglobin Synthesis in Human Fetal Liver Maintained in Short-Term Tissue Culture
- 1 May 1975
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 30 (1) , 9-20
- https://doi.org/10.1111/j.1365-2141.1975.tb00512.x
Abstract
Human fetal liver maintained in tissue culture has been examined as a model system for studying the factors involved in the switch from fetal to adult haemoglobin production. In erythropoietin-treated cultures alpha-, beta- and gamma-chain synthesis remains active for up to 12 days. Globin-chain synthesis is balanced and the alpha/beta + gamma-chain production ratio remains constant up to about 8 days; in longer cultures gamma-chain production declines before that of beta chains. It is concluded that the system may be useful for examining factors involved in the switch from gamma- to beta-chain production provided that these are acting on cells already committed to the erythroid compartment.Keywords
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