Nutritional studies with Pseudomonas aeruginosa grown on inorganic sulfur sources

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Abstract
P. aeruginosa was grown on a succinate-basal salts medium supplemented with various inorganic sulfur compounds as its sole source of S. The organism was able to grow on the sodium salts of sulfide, thiosulfate, tetrathionate, dithionite, metabisulfite, sulfide or sulfate, but not on those of dithionate. Analyses of the culture media after 24 h of growth indicated accumulation of sulfate from each inorganic S source except sulfate. Manometric studies with resting cells obtained by growth on each of these S sources yielded net O2 uptake for all substrates except sulfite and dithionate. Similar results were obtained with extracts from these cells by spectrophotometric techniques. Thiosulfate oxidase activity appeared to be induced by growth on sulfide, thiosulfate or tetrathionate, with little or no activity observed when cells were grown on inorganic S sources of higher oxidative states. Metabisulfite oxidase appeared to be associated with growth on all inorganic S compounds. Rhodanese activity appeared to be constitutively present, and its activity, observed only in soluble fraction, seemed independent of the growth medium employed. Thiosulfate and tetrathionate oxidase activities were studied in greater detail than some of the other sulfur oxidases, and both were distributed between particulate and soluble fractions.

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