THE PREPARATION AND PROPERTIES OF A POLYSACCHARIDE SYNTHESIZED BY ACHROMOBACTER FISCHERI

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Abstract
A polysaccharide extracted from the cells of A. fischeri was shown to serve as a substrate for testicular and streptococcal hyaluronidase. The ability of heat inactivated hyaluronidase to show some depolymerization of the polysaccharide substrate emphasizes the differences between the "hyaluronidase" complex which attacks K hyaluronate and those which are concerned with the breakdown of A. fischeri polysaccharide. The inability of testicular hyaluronidase to inhibit the growth or luminescence of A. fischeri may indicate the existence of the polysaccharide as an endocellular constituent of the cells.