Assay and Some Properties of Kidney Transamidinase.

Abstract
An assay for tissue transamidinase activity was developed. The enzyme was inhibited by sulfhydryl inhibitors both in vivo and in vitro. Canavanine was 3 times as effective as arginine as an amidine donor in vitro. Kidneys from rats fed a protein-free diet for 12 days had only 24% the activity as kidneys from normal rats. The activity could be restored to normal by replacing the protein-free diet with a complete diet.