Genomic Structure and 5′-Flanking Sequences of RatN-Acetylglucosaminyltransferase I Gene and Regulatory Role of Its Transcriptional Diversity
- 1 January 2003
- journal article
- Published by Oxford University Press (OUP) in Bioscience, Biotechnology, and Biochemistry
- Vol. 67 (7) , 1515-1521
- https://doi.org/10.1271/bbb.67.1515
Abstract
It has been known that mouse, rat, and human N-acetylglucosaminyltransferase I (GnT-I) genes produce at least two transcripts, which differ in their 5′-untranslated region (5′-UTR) length, and the longer transcript is preferentially expressed in brains. However, the physiological meaning of this brain-specific expression pattern was unknown. We cloned the rat GnT-I gene and analyzed its structure. It consisted of five exons, and four of them coded only 5′-UTRs. A putative NF-κB binding site was found in the 5′-flanking sequence for the transcript that was previously shown to be induced by inflammation. The unusually long 5′-UTR of the major GnT-I transcript in rat brain was shown to inhibit protein production from the following coding sequence in COS7 cells. Comparison of the GnT-I protein/mRNA ratio in rat brain and liver showed that GnT-I mRNA in the brain was translated 3.8-times less efficiently than in the liver. Implications are discussed of these results in regulation of GnT-I expression in rat brain.Keywords
This publication has 20 references indexed in Scilit:
- Different response to inflammation of the multiple mRNAs of rat N‐acetylglucosaminyltransferase I with variable 5′‐untranslated sequences1FEBS Letters, 1998
- Organization of the human β-1,2-N-acetylglucosaminyltransferase I gene (MGAT1), which controls complex and hybrid N-glycan synthesisBiochemical Journal, 1997
- Essential role for complex N-glycans in forming an organized layer of bronchial epithelium.Proceedings of the National Academy of Sciences, 1996
- Complex asparagine-linked oligosaccharides in Mgat1-null embryosGlycobiology, 1995
- Regulation of N-linked glycosylation. Neuronal cell-specific expression of a 5’ extended transcript from the gene encoding N-acetylglucosaminyltransferase IGlycobiology, 1994
- Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates.Proceedings of the National Academy of Sciences, 1994
- Cloning of a cDNA EncodingN-Acetylglucosaminyltransferase I from Rat Liver and Analysis of Its Expression in Rat TissuesBioscience, Biotechnology, and Biochemistry, 1994
- Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase IGlycobiology, 1992
- Molecular cloning and characterization of the mouse UDP-N-acetylglucosamine:α-3-d-mannoside β-1,2-N-acetylglucosaminyltransferase I geneGenomics, 1992
- Assembly of Asparagine-Linked OligosaccharidesAnnual Review of Biochemistry, 1985