Nucleotide Sequence of a Bovine Protamine cDNA

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Abstract
The nucleotide sequence of a 441-base cDNA encoding the bovine protamine has been determined. This insert, isolated from a bovine spermatid-specific cDNA library, encodes a polypeptide of 50 amino acids of which 26 are arginine, 7 are cysteine, and 2 are tyrosine. The insert contains the complete 3''-noncoding region of 150 bases and most of the 5''-noncoding region. The predicted amino-acid sequence of bovine protamine is about 96% homologous to ram protamine, 76 to boar protamine, 64% to mouse protamine 1 and 52% to human protamine 1 and contains the central, highly basic domain of four arginine clusters found in the trout protamines. Our results show that bovine protamine is 50 amino-acid residues in length and not 47 residues as previously published (Coelingh, J. P. et al. (1972) Biochim. Biophys. Acta 285, 1-14).