Direct Organogenesis from Petiole and Thin Cell Layer Explants in Sugar Beet CulturedIn Vitro

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Abstract
Detrez, C., Tetu, T., Sangwan, R. S. and Sangwan-Norreel, B. S., 1988. Direct organogenesis from petiole and thin cell layer explants in sugar beet cultured in vitro.—J. exp. Bot. 39: 917–926. Plant regeneration was obtained by direct bud formation from petiole as well as from thin cell layer explants taken from sugar beet (Beta vulgaris L.) plants grown in vitro. The buds were mainly induced in the blade-petiole transition zone of the explants. High frequency bud regeneration was observed in petiole and thin layer explants of 10 different breeding lines of sugar beet tested. Organogenesis resulted when petiole explants excised from 8-d-old seedlings grown on half-strength Murashige and Skoog medium (MS) containing 3.0 mg dm−3 naphthalene acetic acid (NAA), 3.0 mg dm−3 6-benzylaminopurine (BAP) and 1.0 mg dm−3 2, 3, 5, triiodobenzoic acid (TIBA) were cultured on MS with 3.0 mg dm−3 NAA and 3.0 mg dm−3 BAP. Thin cell layer strips isolated from shoot apices cultured on MS medium supplemented with 0–9 mg dm−3 BAP or 1.0 mg dm−3 indolebutyric acid (IBA) formed adventitious buds on MS medium containing 0–5 mg dm−3 NAA + 5.0 mg dm−3 BAP. Histological studies confirmed the sub-epidermal origin of shoots.