Biosynthesis of Gangliosides from Asialogangliosides in Rat Liver Golgi Vesicles
- 1 January 1988
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 369 (1) , 55-64
- https://doi.org/10.1515/bchm3.1988.369.1.55
Abstract
Biosynthesis of glycolipids GA2, GA1, GM1b, and GD1c was studied in Golgi vesicles isolated from rat liver. Sequential addition of N-acetylgalactosamine, galactose and two sialic acid residues to lactosylceramide led to the endproduct GD1c. Activities of the corresponding glycosyltransferases were shown to be present in isolated Golgi vesicles and their respective kinetic data were determined. The products of each reaction were characterized by their mobility on thin-layer chromatography, by enzymic degradation of their respective precursors, and in case of GM1b by FAB mass spectrometry.This publication has 17 references indexed in Scilit:
- Oligosialogangliosides Inhibit GM2- and GD3-Synthesis in Isolated Golgi Vesicles from Rat LiverBiological Chemistry Hoppe-Seyler, 1987
- Analysis of gangliosides using fast atom bombardment mass spectrometryChemistry and Physics of Lipids, 1985
- Age- and Hormone-Dependent Ganglioside Patterns of Rat Hepatocytes in Primary CultureBiological Chemistry Hoppe-Seyler, 1985
- Glycosphingolipid Biosynthesis through Asialogangliosides in Rat Bone Marrow Cells1The Journal of Biochemistry, 1982
- Ganglioside Biosynthesis in Rat LiverEuropean Journal of Biochemistry, 1981
- A method for rapid isolation of rough and smooth microsomes and golgi apparatus from rat liver in the same sucrose gradientExperimental Cell Research, 1980
- Ganglioside biosynthesis in rat liver characterization of cytidine-5′-monophospho-n-acetylneuraminic acid: Hematoside (Gm3) sialyltransferaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- A simple and novel method for tritium labeling of gangliosides and other sphingolipidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1978
- Ganglioside biosynthesis characterization of CMP-n-acetylneuraminic acid: Lactosylceramide sialyltransferase in golgi apparatus from rat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- GANGLIOSIDE BIOSYNTHESIS - CHARACTERIZATION OF URIDINE-DIPHOSPHATE GALACTOSE - GM2 GALACTOSYLTRANSFERASE IN GOLGI APPARATUS FROM RAT-LIVER1976