Genetic alterations in potassium transport in L cells.

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Abstract
Starting with mutagenized cultures of the mouse fibroblastic cell line LM(TK-) thymidine kinase deficient, mutant clones were selected by their ability to grow at 0.2 .mu.M, K+, a concentration unable to support the growth of the parent cell. The mutants fall into 2 classes on the basis of their K+ transport properties. Both classes maintain a high intracellular K+ concentration when growing low-K+ medium, and both are unaltered in the ouabain-sensitive Na/K+ pump. One class shows an increased activity of a ouabain-resistant, furosemide-sensitive K+ transport system; the other class shows a decreased activity of a specific component of K+ efflux.