HORMONAL REQUIREMENTS FOR MYOGENESIS OF STRIATED MUSCLE IN VITRO: INSULIN AND SOMATOTROPIN

Abstract
Fusion of presumptive chick embryonic myo-blasts to form myotubes in vitro is almost completely reduced in the absence of animal serum. Addition of insulin greatly stimulates the formation of myotubes, and the further inclusion of somatotropin in the culture medium further enhances, but does not replace, the effect of insulin.