Fish Cell Culture: Characteristics of a Continuous Fibroblastic Cell Line from Walleye (Stizostedion vitreum vitreum)

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Abstract
A cell line (WC-1) was initiated from a dermal tumor from walleye (S. vitreum vitreum). The fibroblastic cells were propagated in Eagle''s minimum essential medium, supplemented with 10% fetal bovine serum, as monolayer cultures that exhibited only slight density-dependent inhibition of mitosis. Cells, which were characterized at passage levels 45-46, grew optimally at 20-25.degree. C, but growth was limited at 10 and 30.degree. C. The line has a chromosome modal number of 48 or 49. Zymograms for lactate dehydrogenase and malate dehydrogenase of WC-1 cells and of normal walleye tissue were very similar, indicating that the WC-1 line may be distinguished by these isozymes. Cell cultures supported replication (with resulting cytopathology) of infectious pancreatic necrosis virus strain VR 299 and the amphibian icosahedral cytoplasmic deoxyribovirus LT-1. The WC-1 line which has now been subcultured more than 100 times can properly be regarded as a continuous cell line.