An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells
- 1 May 1989
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology
- Vol. 25 (5) , 477-480
- https://doi.org/10.1007/bf02624636
Abstract
We have used the cholesterol auxotrophy of NS-1 mouse myeloma cells as the basis for selecting NS-1 hybridomas. The outgrowth of nascent NS-1 hybridomas in cholesterol-free serum-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma wells. This method of hybridoma selection can be applied with any sterol-dependent parent cell line. Hybridomas established under serum-free culture conditions were growth inhibited by fetal calf serum.This publication has 16 references indexed in Scilit:
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