Presence and Regulation of α-Ketoglutarate Dehydrogenase Complex in a Glutamate-Producing Bacterium,Brevibacterium flavum
- 1 August 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 44 (8) , 1897-1904
- https://doi.org/10.1080/00021369.1980.10864219
Abstract
The presence of α-ketoglutarate (α-KG) dehydrogenase complex in the glutamate-producing bacteria was demonstrated for the first time with Brevibacterium flavum. The partially purified enzyme, which was specific to KG and NAD+ with the usual requirements for other co-factors, was labile and stabilized by glycerol, Mg2+, and thiamine pyrophosphate. The enzyme showed an optimum pH of 7.6 and Kms of 80, 86, and 61 μm for KG, NAD+, and CoA, respectively, cis-Aconitate, succinyl-CoA, NADPH, NADH, pyruvate, and oxalacetate strongly inhibited the activity, while it was activated by acetyl-CoA, but not by AMP. Various inorganic and organic salts also inhibited the activity. When cells were cultured in glucose and acetate media, the specific activity of the cell extracts increased markedly and reached to a maximum at the late-logarithmic phase. Then, it decreased to the basal level. The addition of glutamate stimulated the synthesis of the enzyme.This publication has 1 reference indexed in Scilit:
- Effect of Biotin on the Bacterial Formation of Glutamic AcidThe Journal of Biochemistry, 1962