INHIBITION OF PROLIFERATION OF A MURINE MYELOMA CELL-LINE AND MITOGEN-STIMULATED LYMPHOCYTES-B BY THE ANTIBIOTIC AMPHOTERICIN-B (FUNGIZONE)

  • 1 January 1982
    • journal article
    • research article
    • Vol. 47  (1) , 115-121
Abstract
Amphotericin B at the concentration normally used for routine suppression of fungal infection in tissue culture strongly inhibits the proliferation of [mouse] NS1/1 myeloma cells and the LPS[lipopolysaccharide]-induced activation of B lymphocytes from mouse spleen. The proliferation of T lymphocytes induced by concanavalin A (Con A) was less affected by the antibiotic, indicating that B lymphocyte proliferation was preferentially inhibited. The unexpected sensitivity of B lymphoid cells to amphotericin B precludes its use as an anti-fungal agent in the production of hybridomas from fusions between these cells.