A Micropropagation Protocol for Mass Multiplication and Off-Site Conservation ofCelastrus paniculatus-

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Abstract
A protocol was developed using nodal shoot segments as explants for mass/clonal propagation of Celastrus paniculatus-an important threatened medicinal plant. Four to five shoots differentiated from nodal region within 15-20 days on Murashige and Skoog's (MS; 1962) medium containing 1.5 mg 1−1 6-benzylamino purine (BAP) + 0.1 mg 1−1 naphthaleneacetic acid (NAA) + additives (50 mg 1−1 ascorbic acid and 25 mg 1−1 each of adenine sulfate, arginine and citric acid) at 28 ± 2°C temperature and 36 µmol m−28−1 photonflux density, 10 h/day photoperiod. In vitro produced shoots were further multiplied by subculturing on modified Murashige and Skoog's (MMS) medium containing 0.8 mg 1−1 BAP + 0.1 mg 1−1 NAA + additives. Five to seven shoots regenerated from each node of subcultured shoots; on subsequent sub-culturing rates of multiplication were increased to 10-15 folds. The mother explant could also be repeatedly transferred onto fresh medium to yield fresh crops of shoots. After 10-12 culture cycles the cu...

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