Enumeration of rhizobia by enzyme‐linked immunosorbent assay (ELISA)
- 1 February 1985
- journal article
- research article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 58 (2) , 187-193
- https://doi.org/10.1111/j.1365-2672.1985.tb01446.x
Abstract
The use of the enzyme‐linked immunosorbent assay (ELISA) to enumerate rhizobia in peat carrier and in soil has been investigated. The ELISA technique takes less time than the conventional plant infection technique often used to enumerate rhizobia present in the presence of other micro‐organisms. A minimum of 102–103 cells are required for a detectable ELISA reaction, limiting the use of this technique when the number of rhizobia is low.This publication has 11 references indexed in Scilit:
- A Simple Indirect ELISA using F(ab')2 Fragments of ImmunoglobulinJournal of General Virology, 1982
- Immunosorbent Assays in Plant PathologyAnnual Review of Phytopathology, 1981
- A Note on a Highly Sensitive Modified ELISA Technique forRhizobiumStrain IdentificationJournal of Applied Bacteriology, 1980
- Rhizobium strain identification in Arachis hypogaea nodules by enzyme-linked immunosorbent assay (ELISA)Canadian Journal of Microbiology, 1978
- Characteristics of the Microplate Method of Enzyme-Linked Immunosorbent Assay for the Detection of Plant VirusesJournal of General Virology, 1977
- Immunoassay using antigen—enzyme conjugatesFEBS Letters, 1971
- Accuracy of a Plant-Infection Technique for Counting Populations of Rhizobium trifoliiApplied Microbiology, 1963
- Determination of the number of root nodule bacteria in the presence of other organismsAustralian Journal of Experimental Agriculture, 1962
- Estimation of number of root-nodule bacteria by a nodulation-dilution frequency methodSoil Science and Plant Nutrition, 1961
- The Growth Of Rhizobium in Synthetic MediaAustralian Journal of Biological Sciences, 1961