Estimation of number of root-nodule bacteria by a nodulation-dilution frequency method

Abstract
Rhizobia spend most of their life in the soil outside the host plants. Soil conditions, therefore, may exert influence on the survival and variation of rhizobia so as to contribute to the nitrogen fixation of the legumes. The knowledge on the ecology of Rhizobium in soils may expected to give basis for the success of seed inoculation and establishment of effective strains in the field. In addition, it will render service for the study of the ecological soil microbiology, for example, ecology of pathogens of soil-borne plant diseases.

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