Quantitative Determination of Different Apolipoprotein B Containing Lipoproteins by an Enzyme Linked Immunosorbent Assay: Apo B with Apo C-III and Apo B with Apo E
- 1 September 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 12 (3) , 305-323
- https://doi.org/10.1080/01971529108055075
Abstract
A non competitive enzyme-linked immunosorbent assay (ELISA) for total apolipoprotein (apo) B, apo B with apo C-III (LpC-III:B), and apo B with apo E (LpE:B), was developed. Microtiter plates were used as solid-phase, and subdivided into three parts, coated respectively with affinity purified antibodies to apo B, to apo C-III and to apo E. After incubating the antigen with coated plates, a horseradish peroxidase-labelled antibody to apo B was added to all the plates to estimate total apo B, LpC-III:B and LpE:B.Keywords
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