A study on the immunological response in chickens to the somatic antigen of Salmonella gallinarum.

  • 1 November 1968
    • journal article
    • Vol. 15  (5) , 653-60
Abstract
The immune response in chickens to the O antigen of Salmonella gallinarum has been studied. Chickens were infected orally with Salmonella gallinarum organisms, inoculated intramuscularly (i.m.) with heat-killed organisms, both alone and in Freund's complete adjuvant, and inoculated i.m. with a Westphaltype lipopolysaccharide extract of the organism, administered both alone and in Freund's complete adjuvant. Serum was collected at intervals and fractionated in Sephadex G-200 gel and by linear sucrose density ultracentrifugation. The fractions were examined by bacterial agglutination, indirect haemagglutination and antiglobulin haemagglutination tests.