PREPARATION AND PROPERTIES OF A STANDARDIZED LIPOPOLYSACCHARIDE FROM SALMONELLA ABORTUS EQUI (NOVO-PYREXAL)

  • 1 January 1979
    • journal article
    • research article
    • Vol. 243  (2-3) , 226-244
Abstract
The preparation of the lipopolysaccharide from S. abortus-equi as obtained by standardized methods is described. They include extraction with phenol/water followed by phenol/chloroform/petroleum ether extraction, ultra-centrifugation, electrodialysis and conversion to a uniform sodium salt form. Chemical composition and physico-chemical properties are described. The preparation, which is free of contaminants, was tested for local Schwartzman reactivity, pyrogenicity, lethal toxicity, mitogenicity, reactivity towards complement and tumoricidal action.