MODE OF ACTION OF THE HYPERGLYCEMICGLYCOGENOLYTIC FACTOR FROM URINE1

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Abstract
The intravenous administration to rabbits of a urinary extract prepared from normal human urine produces hyperglycemia. The doses employed (1 or 5 mg/kg. body weight) correspond to a small fraction of the daily output by normal individuals. The urinary extract stimulates glycogenolysis by liver homogenates, and in sufficiently high doses (100 [mu]g/50 mg of tissue) induces complete disappearance of tissue glycogen. Evidence is presented indicating that at least part of the increased glycogenolysis found in homogenates treated with the urinary extract is not due to stimulation of phosphorylase, amylo-l,6-glucosidase, phospho-glucomutase or glucose-6-phosphate phosphatase. Fluoride (0.1 [image]), which completely inhibits the increased glycogenolysis elicited by glucagon in liver slices, does not completely inhibit the glycogenolytic action of the urinary extract. The extract was shown to contain urinary amylase. It is tentatively concluded that serum amylase may influence the concentration of hepatic glycogen in physiological or pathological states.

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