SENSITIVE AND SPECIFIC DETERMINATION OF THE NEW ANTHRACYCLINE ANALOG 4'-EPIDOXORUBICIN AND ITS METABOLITES BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 12  (1) , 9-13
Abstract
A sensitive and specific method for the determination of 4''-epidoxyorubucin [an antitumor agent] and its metabolites in biological fluids was developed which uses reverse phase high pressure liquid chromatography and fluorescence detection. Daunorubicin was used as an internal standard. The limit of detection for 4''-epidoxorubicin and 4''-epidoxorubicinol was 1 ng/injection, and concentrations as low as 5 ng/ml could be analyzed in plasma and urine. Preliminary pharmacokinetic studies were performed on 3 patients receiving a 15-min infusion of 4''-epidoxorubicin, one at a dose of 50 mg/m2 and two at 60 mg/m2. A .beta.-glucuronide of 4''-epidoxorubicin was identified as a major metabolite in urine. Significant aglycone formation was found in plasma.