Genomic Fingerprinting of Two Pathovars of Phytopathogenic Bacteria by Rare-Cutting Restriction Enzymes and Field Inversion Gel Electrophoresis
- 1 January 1989
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 79 (7) , 745-750
- https://doi.org/10.1094/phyto-79-745
Abstract
Several restriction endonucleases cut infrequently in the genomes of Pseudomonas syringae and pathovars of Xanthomonas campestris, and distinctive patterns of large DNA fragments were generated using field inversion gel electrophoresis. The resolution of genomic DNA fragments by this method permitted standard analyses of genetic relationships between strains by means of dissimilarity coefficients and cluster analyses. We examined 10 strains of P. s. tomato and and identified two groups that could be readily distinguished from each other. Strains of other pathovars of P. syringae had genomic fingerprints very different from those of the strains of X. c. vesicatoria all had similar genomic fingerprints, but distinctive subgroups within the pathovar could be identified. Strains of other pathovars of X. campestris also had very different genomic fingerprints from the strains of X. c. vesicatoria.This publication has 29 references indexed in Scilit:
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