Abstract
The sugars and amino acids which occur in an uncombined state in the barley corn have been separated by means of paper partition chromatography. In addition to the sugars known to be present in ungerminated barley, viz., raffinose, sucrose, glucose, fructose and a trace of maltose, there is present an oligosaccharide which proves to be slightly more mobile than raffinose in most of the solvents used, and which can be hydrolysed to yield only glucose and fructose. The amino acids and amides present include α-alanine, arginine, aspartic acid, glutamic acid, glycine, leucine and froleudne, lysine, phenylalanine, proline, serine, valine, asparagine and a trace of glutamine. γ-Amino-butyric acid has been identified provisionally. Two ninhydrin-reacting compounds remain unidentified.