α-DNA IX: parallel annealing of α-anomeric oligodeoxyribonucleotides to natural mRNA is required for interference in RNase H mediated hydrolysis and reverse transcription
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (13) , 5107-5115
- https://doi.org/10.1093/nar/17.13.5107
Abstract
Ps- and aps-.alpha. anomeric oligodeoxyribonucleotides were designed to recognize in parallel (ps) or antiparallel (aps) orientation two different sites of a 1000 base-long mRNA. Northern blots experiments indicate that only ps-.apprx.-oligonucleotides were able to hybridize to the mRNA target. Furthermore, only ps-.alpha.-oligonucleotides were able, in a sequence specific way, to protect the mRNA target against RNase H mediated hydrolysis or to inactivate the priming capacity of .beta.-oligodeoxynucleotide probes in reverse transcription. Formation of parallel-stranded mRNA .alpha.-oligonucleotide miniduplexes which prevents hybridization of .beta.-oligonucleotide probes is the most likely mechanism accounting for these results. Use of .alpha.-oligonucleotides as potential gene control agents is discussed.This publication has 23 references indexed in Scilit:
- Parallel Stranded DNAScience, 1988
- α-DNA VII. Solid phase synthesis of α-anomeric oligodeoxyribonucleotidesNucleic Acids Research, 1988
- Parallel stranded duplex DNANucleic Acids Research, 1988
- Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleoside methylpbosphonates in a cell-free systemNucleic Acids Research, 1988
- A new approach to chemotherapy based on molecular biology and nucleic acid chemistry: Matagen (masking tape for gene expression).1987
- Redefining interferon: the interferon-like antiviral effects of certain cytokines (interleukin-1, interferon-β2, interferon-γ) may be indirect or side effectsAntiviral Research, 1987
- α-DNA VI: comparative study of α- and β-anomeric oligodeoxyribonucleotides in hybridization to mRNA and in cell free translation inhibitionNucleic Acids Research, 1987
- 2D-NMR studies of the unnatural duplex α-d(TCTAAAC)-β-d(AGATTTG)Nucleic Acids Research, 1987
- α-DNA IV: α-anomeric and β-anomeric tetrathymidylates covalently linked to intercalating oxazolopyridocarbazole. Synthesis, physicochemical properties and poly (rA) bindingNucleic Acids Research, 1987
- α-DNA-V. Parallel annealing, handedness and conformation of the duplex of the unnatural α-hexadeoxyribonucleotide α-[d(CpApTpGpCpG)] with its β-complement β-[d(GpTpApCpGpC)] deduced from high field1H-NMRNucleic Acids Research, 1987