Physicochemical characterization and isolation of rabbit kidney-specific autoantigens.

  • 1 May 1973
    • journal article
    • Vol. 24  (5) , 911-24
Abstract
Rabbit kidney tissue contains antigens which are tissue-specific and species restricted, as well as other antigens which are shared by different organs of the animal. The stability of these antigens was investigated as a function of temperature, in order to explore the possibility of thermal fractionation. It was observed that one rabbit kidney-specific autoantigen was destroyed at 56° and another at 65°. A third antigen, which is restricted to the rabbit species but is non-tissue specific, was destroyed at 72°. Ultracentrifugal analysis of the saline extract at different concentrations showed the presence of several components, whose extrapolated values at zero concentration were found to be 4.2S, 6.2S, 10S, 19S, and 80S. The first two components were the most prominent.