Plant regeneration from protoplasts of wild rice (Oryza rufipogon Griff.)
- 1 July 1991
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 10 (4) , 200-203
- https://doi.org/10.1007/bf00234295
Abstract
Embryogenic callus initiated from basal segments of micropropagated shoots of Oryza rufipogon were used to initiate cell suspension cultures. After approximately 3 months these cultures were capable of yielding large numbers of protoplasts which underwent sustained division in agarose-solidified medium at a frequency comparable to that observed with Japonica rice protoplasts in previous studies. O. rufipogon plants were reproducibly regenerated from the protoplast-derived callus and are currently being grown to maturity. This is the first report of plant regeneration from protoplasts of a wild species of Oryza.Keywords
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