Identification of a receptor protein for neuropeptide Y in rabbit kidney G‐protein association and inhibition of adenylate cyclase
- 25 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (2) , 219-222
- https://doi.org/10.1016/0014-5793(91)80153-t
Abstract
We previously identified the receptor for neuropeptide Y (NPY) on rabbit kidney membranes as a 100 kDa protein [(1990) J. Biol. Chem. 265, 18142–18143]. It is demonstrated in this study that the stable guanine nucleotide analogue, guanosine 5′‐O‐(3‐thiotriphosphate)(GTPγS), is capable of decreasing the labeling of the 100 kDa protein in a concentration‐dependent manner, with an IC20 value of 50 μM. This suggests that the 100 kDa protein represents a G‐protein coupled receptor type. In isolated proximal tubulex from rabbit kidney NPY decreases the parathyroid hormone stimulated cAMP production in a dose‐dependent fashion. This indicates that the NPY receptor in rabbit kidney is negatively coupled to adenylate cyclase by a G1‐like protein.Keywords
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