Expression and quantitative changes of carbonic anhydrase in developing neurones of rat central nervous system

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Abstract
Postnatal changes in carbonic anhydrase activity were investigated in the islands of Calleja, which have been previously reported to contain the enzyme. Results obtained with a new modified method of Hansson provided further evidence for the distinction between the medial and lateral islands of Calleja. The enzyme was localized mainly in the nucleus and cytoplasm of granule cells without showing binding to any cytoplasmic organelle. No large neurons of the islands displayed carbonic anhydrase reactivity. The time course and rate of increase of carbonic anhydrase expression were different in the giant island of Calleja and lateral islands and this finding may strengthen the hypothesis regarding the medio-lateral diversity of Calleja's islands. On the other hand, at the end of the maturation process the granule cell complexes showed no significant difference in the proportion of carbonic anhydrase positive neurones. The almost equal rate of appearance of carbonic anhydrase reactive granule cells raises the possibility of a basic common role of both medial and lateral islets.