Characterization of RNA polymerase type II from human term placenta
- 1 June 1986
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 127 (3) , 432-438
- https://doi.org/10.1002/jcp.1041270312
Abstract
RNA polymerase type II from human term placenta has been isolated and characterized with respect to its template, ammonium sulfate, divalent cation, and buffer preferences. In addition, the apparent Michaelis constants for AMP amd UMP incorporation have been determined. The enzyme was also analyzed by native and denaturing polyacrylamide gel electrophoresis, and evidence is presented that a single polypeptide is radiolabeled with azido purine nucleoside triphosphate photoprobes.This publication has 32 references indexed in Scilit:
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