Studies on transfer ribonucleic acids and related compounds. XLV. Block condensation of ribooligonucleotides containing 2' -O-tetrahydrofuranyl-5' -O-dimethoxytritylnucleosides
Open Access
- 11 March 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (5) , 1325-1335
- https://doi.org/10.1093/nar/11.5.1325
Abstract
2′-O-Tetrahydrofurany1-5′-O-dimethoxytrityl-N-protected nucleosides were phosphorylated to give the 3′-(o-chlorophenyl) phosphates which were then condensed with 3′,5′-unprotected nucleosides to elongate the chain in the 3′-direction. The 5′-dimethoxytrityl group of these oligonucleotides was selectively deblocked by treatment with zinc bromide. The rate of removal of the dimethoxytrityl group differed in each nucleotide. A dodecamer containing a termination codon UAG, U(AGU)3,AG, was synthesized by elongating the chain in the 5′-direction using the selective dedimethoxytritylation followed by condensation of protected oligonucleotide blocks.Keywords
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