Ascorbic acid determination in serum and aqueous humour by high-performance liquid chromatography

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Abstract
High-performance liquid chromatography on a Supelcosil LC-HN2 analytical column in weak anion exchange model has allowed separation of ascorbic acid, in < 5 min, from other interfering substances in serum and aqueous humor. UV monitoring at 254 nm enables ascorbic acid to be detected at 20 pmol level. A method for determination of ascorbic acid concentration in serum and aqueous humor is described, and values from 10 cataract patients are reported.