Comparison of seven enzyme immunoassay systems for measurement of cytomegalovirus

Publisher Website
Google Scholar
Abstract
The relative sensitivities of 7 different enzyme immunoassay (EIA) systems for the measurement of cytomegalovirus (CMV) were compared. Methods which used 2 separate antisera to CMV provided the greatest degree of sensitivity. equivalent sensitivity was noted with the use of either enzyme-labeled antiglobulin or unlabeled staphylococcal protein A and rabbit enzyme-antienzyme complex to measure the second anti-CMV antibody bound to the solid phase. Single-antibody methods were less sensitive than the double-antibody methods but were more sensitive than an inhibition EIA. The sensitivity of the inhibition EIA was improved when CMV-antibody complexes were separated from unreacted antibody by precipitation with polyethylene glycol. Double-antibody EIA systems are preferable when antisera prepared in 2 different animal species are obtainable. A number of single-antibody EIA systems can be formulated for use in situations where only a single antiserum is available.