Adenine Nucleotides and Glycolysis during Liver Preservation by Simple Hypothermic Storage

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Abstract
Adenine nucleotides and glycolytic metabolites were examined during hypothermic storage of porcine livers by different preservation methods. The parameters ATP, ADP, AMP, G6P, F6P, FDP, GAP, 2PG, PEP, Pyr and Lact were investigated. The results indicate a dependence of adenine nucleotides but no dependence of glycolytic metabolites on preservation method. Storage by plasma protein fraction or Collins C2 solution causes a better result in TAN concentration after 6 h than albumin and Ringer storage or Ringer trickle perfusion. Conditioning the organ for a better outcome of preservation aimed at increasing the glycolytic rate is of questionable value.

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