Peptide YY Stimulates the Expression of Apolipoprotein A-IV Gene in Caco-2 Intestinal Cells
- 1 March 2000
- journal article
- Published by Wiley in Proceedings of the Society for Experimental Biology and Medicine
- Vol. 223 (3) , 270-275
- https://doi.org/10.1046/j.1525-1373.2000.22338.x
Abstract
The effect of peptide YY, a gastrointestinal hormone, on the expression of the apolipoprotein A-IV gene in the intestinal epithelial cell line Caco-2 was examined by semiquantitative RT-PCR followed by Southern hybridization with an inner oligonucleotide probe. Apolipoprotein A-IV mRNA levels were increased in response to peptide YY in a dose- and time-dependent fashion. Western blotting revealed that the exogenous peptide YY increased the intracellular concentration of apolipoprotein A-IV. In contrast, apolipoprotein A-I, B, and C-III mRNA did not respond to peptide YY. Differentiated Caco-2 cells expressed Y1- but not Y2- and Y5-receptor subtype mRNA. The present results suggest that peptide YY modulates apolipoprotein A-IV gene expression, likely via the Y1-receptor subtype in intestinal epithelial cells.Keywords
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