Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization.

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Abstract
The growth of adenovirus type 12 in African green monkey kidney was significantly enhanced by SV40 virus preinfection as indicated by the development of increased virus infectivity and CF antigen. After 6 tissue culture sub-passages, the oncogenicity of the resulting virus for newborn hamsters was also remarkably potentiated and accelerated. The potentiation of oncogenicity was not due to a mere mixing of SV40 virus and adenovirus particles but developed only after additional growth of the 2 viruses together for several subcultures. Tumor antigens characteristic of both viruses were demonstrated in all primary tumors induced by the postulated hybrid virus and remained present in tumors carried through 5 transplant passages. The oncogenic and T antigen determinants were eliminated by adenovirus type 12 antiserum but not by antiserum to SV40 virus, thus suggesting that SV40 genetic information was contained in some of the adenovirus capsids.