Analytical Ultracentrifugation for Characterizing Nanocrystals and Their Bioconjugates
- 6 April 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 5 (5) , 963-967
- https://doi.org/10.1021/nl047926f
Abstract
Analytical ultracentrifugation (AU) provides a general way to probe the polydispersity of nanoparticles and the formation of bioconjugates in solution. Unconjugated gold nanocrystals show sedimentation coefficient distributions that are in agreement with size distributions as measured by TEM. AU is sensitive to the size/shape changes elicited by conjugation, in this case to lactose repressor (LacI). AU data reveal saturating protein concentrations for conjugates that correspond to the measured stoichiometry of the complex under these conditions.Keywords
This publication has 26 references indexed in Scilit:
- Nanocrystal Surface Structure Analysis by Analytical UltracentrifugationLangmuir, 2003
- Conformation of Oligonucleotides Attached to Gold Nanocrystals Probed by Gel ElectrophoresisNano Letters, 2002
- Electrophoretic and Structural Studies of DNA-Directed Au Nanoparticle GroupingsThe Journal of Physical Chemistry B, 2002
- Efficient Near-Infrared Polymer Nanocrystal Light-Emitting DiodesScience, 2002
- Albumin−CdTe Nanoparticle Bioconjugates: Preparation, Structure, and Interunit Energy Transfer with Antenna EffectNano Letters, 2001
- DNA-Based Assembly of Gold NanocrystalsAngewandte Chemie International Edition in English, 1999
- High-Resolution, Submicron Particle Size Distribution Analysis Using Gravitational-Sweep SedimentationBiophysical Journal, 1999
- Semiconductor Nanocrystals as Fluorescent Biological LabelsScience, 1998
- One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle ProbesJournal of the American Chemical Society, 1998
- Organization of 'nanocrystal molecules' using DNANature, 1996