Rat liver plasma membranes contain a lipid‐dependent protein kinase activity
- 1 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 148 (1) , 131-134
- https://doi.org/10.1016/0014-5793(82)81258-1
Abstract
The treatment of rat liver plasma membranes with EDTA resulted in substantial release of a protein kinase activity. Using histones as external substrates both phosphatidylserine (PS) and diolein activated the enzyme. The activatory effect of PS but not that of diolein depended on Ca2+. In contrast, the enzyme activity was inhibited by both PS and diolein when the substrate was protamine. The inhibition by PS, unlike by diolein, depended on Ca2+. Data suggest that mobilization of plasma membrane-bound Ca2+ by physiological stimuli may be accompanied by release of the lipid-dependent protein kinase into the cytoplasm.Keywords
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