INDIUM-111-LABELED HUMAN POLYMORPHONUCLEAR LEUKOCYTES - VIABILITY, RANDOM MIGRATION, CHEMOTAXIS, BACTERICIDAL CAPACITY, AND ULTRASTRUCTURE
- 1 January 1979
- journal article
- research article
- Vol. 20 (7) , 741-747
Abstract
Human polymorphonuclear leukocytes (PMN) were labeled with 111In oxine in ethanol and the effects of the labeling procedure, radioactivity and concentrations of oxine and ethanol on PMN function and structure were studied in vitro. The standard labeling procedure did not alter the viability, random migration, chemotaxis, bactericidal capacity or the ultrastructure of PMN. Exposure to higher doses of radioactivity or to higher concentrations of ethanol had no appreciable effects on random migration and chemotaxis of PMN. A dose-dependent reduction in their random migration and chemotaxis was observed when higher concentrations of oxine were used. 111In-labeled PMN were apparently structurally intact and had normal in vitro locomotion and bactericidal activity. 111In-labeled PMN should be suitable for studying the kinetics and distribution of these cells in health and disease.This publication has 8 references indexed in Scilit:
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