Effect of Ribosome Binding Site on Gene Expression inEscherichia coli
- 1 April 1988
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 7 (3) , 173-179
- https://doi.org/10.1089/dna.1988.7.173
Abstract
Using the expression of human renin gene in Escherichia coli as a model, we have observed that a distance of 7-10 nucleotides between the Shine–Dalgarno site to the initiation codon ATG is optimal for translation initiation. The sequence ATA as the triplet preceding the ATG gives the best expression among those tested. These observations agree with the statistical bias observed for the genes of E. coli and its phages. We have also compared gene expression with three different Shine–Dalgarno sites. Expression of a given gene can be increased by as much as 1000-fold with slight modifications in the Shine–Dalgarno sequence.Keywords
This publication has 17 references indexed in Scilit:
- High-level expression of human renin in Escherichia coliJournal of Biotechnology, 1986
- Synthesis of Bovine Prolactin inEscherichia coliDNA, 1986
- The effects of hybrid ribosome-binding-site variants on the expression of human Interferon-β in Escherichia coliGene, 1985
- Mutations upstream of the ribosome-binding site affect translational efficiencyJournal of Molecular Biology, 1985
- Alterations upstream from the Shine-Dalgarno region and their effect on bacterial gene expressionGene, 1985
- lacZ translation initiation mutationsJournal of Molecular Biology, 1984
- Efficient Bacterial Expression of Bovine and Porcine Growth HormonesDNA, 1983
- Increased Synthesis inE. coliof Fibroblast and Leukocyte Interferons Through Alterations in Ribosome Binding SitesDNA, 1982
- A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322Gene, 1981
- Translational Initiation in ProkaryotesAnnual Review of Microbiology, 1981