CYTOGENETIC EFFECTS PRODUCED BY NEUTRONS IN LYMPHOCYTES OF HUMAN PERIPHERAL-BLOOD INVITRO .1. DEPENDENCE OF THE EFFECT ON THE DOSE OF NEUTRONS OF DIFFERENT ENERGIES FOR DIFFERENT TYPES OF CHROMOSOME-ABERRATIONS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 15  (6) , 1046-1060
Abstract
Human lymphocytes were irradiated in vitro during the Go stage by graded doses of thermal neutrons and of neutrons with mean energy of 0.04, 0.09, 0.35, 0.85 and 14.7 MeV and by 60Co .gamma.-rays. Data were fitted to linear and linear-quadratic relations. The neutrons of low and intermediate energies showed linear dependence on dose, while 14.7 MeV neutrons and .gamma.-rays showed a linear-quadratic dose dependence. Data obtained with 0.85 MeV neutrons fitted both models well. Terminal and interstitial deletions produced by .gamma.-rays and neutrons showed different dependencies on dose. Some qualitative peculiarities of aberration spectra were found in the experiments with neutrons as compared with the data on .gamma.-irradiation: the ratio of exchanges to fragments was greater, and aberrations of chromatid type were produced. Specially designed experiments and calculations showed that chromatid aberrations were not connected with induced radioactivity.