Synthesis and NMR study of ribooligonucleotides forming a hammerhead-type RNA enzyme system
Open Access
- 25 October 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (20) , 5955-5960
- https://doi.org/10.1093/nar/18.20.5955
Abstract
We designed a hammerhead-type ribozyme system which consists of three RNA fragments and synthesized the component andrelated ribooligonucleotides by the solid-phase phosphoramidite method using o-nitrobenzyl groups for 2′-hydroxyl protection. Improved conditions for photolytic removal of the o-nitrobenzyl groups are described. Imino proton NMR spectra of the ribozyme complex were measured. The resonances for the hydrogen-bonded imino protons were assigned by comparison with the spectra of model complexes which contain the base sequences of one or two of the stem regions. The results suggest that the complex indeed forms a hammerhead structure and the loop regions take an ordered conformation in the absence of magnesium ions.Keywords
This publication has 11 references indexed in Scilit:
- The role of a conserved guanosine residue in the hammerhead‐type RNA enzymeFEBS Letters, 1990
- Solution conformation of an RNA hairpin loopBiochemistry, 1990
- Solid-phase synthesis and high-resolution NMR studies of two synthetic double-helical RNA dodecamers: r(CGCGAAUUCGCG) and r(CGCGUAUACGCG)Biochemistry, 1989
- Construction of a series of several self‐cleaving RNA duplexes using synthetic 21‐mersFEBS Letters, 1988
- A small catalytic oligoribonucleotideNature, 1987
- The Chemistry of Self-Splicing RNA and RNA EnzymesScience, 1987
- Improved synthesis of (Pr21N)2POCH2CH2CNNucleic Acids Research, 1987
- Satellite tobacco ringspot virus RNA: A subset of the RNA sequence is sufficient for autolytic processingProceedings of the National Academy of Sciences, 1986
- Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2′-hydroxyl via a phosphite triester approachNucleic Acids Research, 1986
- Studies on transfer ribonucleic acids and related compounds. IX(1)Ribo-oligonucleotide synthesis using a photosensitive o-nitrobenzyl protection at the 2′-hydroxyl groupNucleic Acids Research, 1974