SOME FACTORS INFLUENCING THE ESTIMATION OF URINARY 17-KETOGENIC STEROIDS

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Abstract
Some of the variables in Norymberski''s method for the estimation of 17-ketogenic steroids were studied. This method consists in the oxidative fission of the corticosteroid side chain with sodium bismuthate producing 17-ketosteroids. Oxidation of 8 ml urine specimens with 3 g of sodium bismuthate for 18 hours gave a significantly higher yield of 17-ketogenic steroids than oxidation with 5 g of bismuthate for half an hour. The principal 17-ketosteroid formed was 11-ketoetiocholanolone. The 24-hour_excretion of 17-ketogenic steroids in 20 healthy women (17-47 years) varied from 6.9 to 17.9 mg dehydro-epiandrosterone-equivalent. Corresponding values in 17 healthy men (22-37 years) were between 11.1 and 22.1 mg. Thus, the excretion by normal men was significantly greater than that by normal women.