CHEMICAL CHANGES IN THE GROWTH MEDIUM OFMICROCYSTIS, BATCH CULTURES GROWN AT STRESS AND NON-STRESS LIGHT INTENSITIES

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Abstract
The modified BG 11 growth medium used in this study has a poor buffer ability and large pH changes occurred during growth of Microcystis. Inhibition of growth in cultures grown at stress light intensity (26 μEinst m−2 sec−1) could be ascribed to depletion of the total inorganic carbon content of the medium rather than to light intensity per se, pH or high O2 concentration. In cell suspensions grown under stress light intensity an unfavourably low CO2: O2 concentration ratio developed due to rapid photosynthesis and insufficient replenishment of CO2 by diffusion from air which may have induced oxygenation instead of carboxylation by ribulose diphosphate carboxylase.