POST‐TRANSCRIPTIONAL DOWNREGULATION OF MHC CLASS I EXPRESSION IN ONCOGENE‐TRANSFORMED CELLS IS REVERTED BY IFN‐GAMMA AND TNF‐ALPHA
- 1 August 1989
- journal article
- research article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 16 (4-5) , 315-320
- https://doi.org/10.1111/j.1744-313x.1989.tb00477.x
Abstract
Transformation of murine NIH3T3 fibroblasts with retroviral vectors carrying the mos, myc and the Ha-ras oncogene, respectively, was associated with a strong reduction of H2 antigen expression in the cell membrane. Analysis of H-2K and .beta.2-microglobulin promoter-driven CAT activity in these oncogenic transformations and normal NIH3T3 fibroblasts revealed unchanged promoter activity, suggesting post-transcriptional control of MHC class I expression by these oncogenes. Treatment with IFN-gamma and TNF-alpha caused 2- to 3-fold enhancements of H-2K and .beta.2-microglobulin promoter activity, as well as a normalization (TNF-alpha treatment) or enhancement (IFN-gamma treatment) of H2 membrane expression. These data suggest that IFN-gamma as well as TNF-alpha can counteract downregulation of H-2 genes by interference with an oncogene-induced, post-transcriptional block as well as by a direct enhancement of H-2 gene transcription.This publication has 16 references indexed in Scilit:
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