Chloramphenicol releases a block in initiation of chromosome replication in a dnaA strain of Escherichia coli K12
- 1 May 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 178 (2) , 329-336
- https://doi.org/10.1007/bf00270480
Abstract
DNA-DNA hybridisation experiments show that chloramphenicol induces a burst of initiation from the oriC region of a dnaA46 mutant of Escherichia coli at 36.5° C but not from the isogenic dnaA + strain. Following this stimulation of initiation, DNA replication proceeds normally towards the terminus. The temporal pattern of the extra initiation is in parallel with the induced stimulation of RNA synthesis caused by chloramphenicol in the same strain. This is consistent with the hypothesis that the stimulation of initiation in the dnaA mutant is the result of the stimulation of the synthesis of an RNA species.This publication has 57 references indexed in Scilit:
- Cloning of replication origins from the E. coli K12 chromosomeNature, 1978
- Purification and characterization of covalently closed replicative intermediates of ColEl DNA from Escherichia coliBiochemistry, 1977
- A suitable method for construction and cloning hybrid plasmids containingEcoRI-fragments ofE. coli genomeMolecular Genetics and Genomics, 1977
- A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genomeCell, 1976
- A simple method for the preparation of large quantities of pure plasmid DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Origin and sequence of chromosome replication in Escherichia coliJournal of Molecular Biology, 1972
- Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T−Journal of Molecular Biology, 1972
- Factor Necessary for Ribosomal RNA SynthesisNature, 1970
- Thermosensitive Mutants of E. coli Affected in the Processes of DNA Synthesis and Cellular DivisionCold Spring Harbor Symposia on Quantitative Biology, 1968
- An Improved Diphenylamine Method for the Estimation of Deoxyribonucleic AcidNature, 1965