The Lysosomal Nature of the Anomalous Granules and Chromosome Aberrations in Cultures of Peripheral Blood in Chediak‐Higashi Syndrome

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Abstract
In Chediak‐Higashi syndrome (C.H.) the lysosomal nature of the giant granules in white blood cells was demonstrated by their strong fluorescence with acridine orange and by their strong acid phosphatase and esterase activities.Cytochemical observations on normal peripheral blood stained for esterase activity in the presence of Triton X‐100 showed an increased activity equal to that found in leucocytes from C.H. cases. This may be due to a modification of a lipid component of the lysosomal membrane.Cultured lymphocytes from C.H. are capable of transformation like normal lymphocytes. The transformation was found not to change the appearance of the inclusion bodies. Although the chromosome number was normal, morphological abnormalities were observed, of which chromatid and chromosome breakages were the most prevalent.