Mutagenesis of Clostridium acetobutylicum

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Abstract
Mutagenesis of the obligate anaerobe C. acetobutylicum was best accomplished using agents (e.g, ethyl methane sulfonate or N-methyl-N''-nitro-N-nitrosoguanidine) which are believed to act by a direct mutagenic mechanism. Other agents (e.g., UV radiation) whose effectiveness relies on misrepair of damaged DNA via an error-prone pathway were poor mutagens of this organism. Procedures are described which readily yielded a variety of auxotrophic and other useful mutant strains of C. acetobutylicum and related saccharolytic clostridia.