Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection

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Abstract
The smooth lipopolysaccharide complex of the outer surface of smooth B. abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smooth lipopolysaccharide complex. The Brucella organism contains a large number of other antigenically distinct components. The biological significance of some of these antigens was demonstrated by showing that sera from infected cattle have precipitins to these components. These sera revealed up to 7 distinct lines in immunoelectrophoresis with a protein-rich antigen mixture prepared from rough strain B. abortus 45/20, whereas sera from strain 19-vaccinated cattle did not reveal these lines at 4 or more months after vaccination. Monospecific antisera were prepared against 6 antigens in this mixture, and the purification of 2 of them by antibody affinity chromatography is described.